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pintoarabian
Gold Member
Scotland
1242 Posts |
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Gerri
Platinum Member
England
4211 Posts |
 Posted - 12 Feb 2010 : 10:15:23 AM
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Wow this is the best thread I have ever read seriously interesting educational and thank you so much for the pictures, it is brilliant, Fab Topic and everyones posts are so informative, thank you so much for sharing it |
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Pashon2001
Platinum Member
3575 Posts |
Posted - 12 Feb 2010 : 4:47:02 PM
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| Gel fraction and seminal fluid are not the same thing. I use a filter to remove the gel fraction, but a centrifuge to remove the seminal fluid, which is the watery substance the sperm is actually swimming in. Then the sperm are removed from the test tube in a very highly concentrated state and reintroduced to extender only, thus giving the same 'dose' but in extender instead of the original fluid. This is mainly for shipping purposes. A similar method is also used for freezing which is why straws have a much higher concentrate of sperm and you don't need as much volume. |
     
www.jarvastud.com http://hocon.webs.com/ |
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TOOTHLESS
Silver Member
Australia
350 Posts |
Posted - 12 Feb 2010 : 4:54:34 PM
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33yrs ago, I started my first horse, a 2yr old pure arabian colt with a mare that stood like a rock until he found the correct place. A few years later I would let him loose in the paddock with the visiting mare, and he would wait until I had hold of the front end of the mare, and only when he felt I had control of her would he approach to cover her. He did this in a very deliberate way and if she squealed he backed of at a rate of knots. I think he learnt the hard way as a result of me allowing others to stand him and use him, and even though he was beautifully mannered they abused him. On one occasion I visited him at the stud he was at and discovered that the tissue between the lower left canine tooth and the first molar had been stripped off the bone. This happened because the Know All, Stupid, supposedly experienced, stud owner handling him used a " bull bit " on him (because he refused to cover a mare)despite me telling him that a leather halter would suffice. That person is still, around, still passing himself off as an expert, still "breeding or trying to breed the perfect arabian", and still as pig- headed as he was all those years ago.
Talquah Taaj ( Allbreeds pedigree) was a dream to handle ( it was just him, me and the mare).I had no fear of him hurting me, or the mare. I make him sound like a dull none entity, but take him to a show and he was awash with presence (it was in his lines), but always controllable,I could have used a string. Sorry , I got sidetracked |
Edited by - TOOTHLESS on 12 Feb 2010 4:58:46 PM |
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Delyth
Gold Member
United Kingdom
1425 Posts |
Posted - 12 Feb 2010 : 7:09:48 PM
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There was speculation of the seminal fluid actually being bad for the sperm and it actually killing it but I could never get my head round that cos it's what nature produces for the sperm ! Gleannloch Farm used a centrifuge many years ago to seperate semen into 'filly' and 'colt' sperm and it worked - they used the wrong half apparantly and got all colts !!
One of the girls at Umm Qarn wrote a paper on gel and it's properties as a treatment for infection in mares I think. I often wonder what happened to Catherine at Umm Qarn she was very knowledgable, she went to work on a TB stud farm in Oz I think ?? |
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Pashon2001
Platinum Member
3575 Posts |
Posted - 12 Feb 2010 : 7:49:23 PM
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I must say that there is definate data on seminal fluid being bad for SOME sperm, and in those cases removing it has extended and enhanced certain horses semen. I must say that I have first hand experience that it works. I knew of one stallion thats semen lived only hours un centrifuged and days if seperated. The filly and colt thing does work to a certain extent as one is heavier than the other so can be seperated ( not sure which way round it is).
I was told that the gel being the last part of the ejeculate that the stallion produces is there to 'plug' the uterus (not literally) and also act as a barrier against another boys advances. Hence the reason for removing it when collecting so it doesnt get mixed in. There were some very knowledgeable people at UMM Qarn, shame it isnt still here. They were my first experiences with AI. |
www.jarvastud.com http://hocon.webs.com/ |
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Delyth
Gold Member
United Kingdom
1425 Posts |
Posted - 12 Feb 2010 : 8:16:50 PM
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| Now you've got me wondering. I've been looking at centrifuge.org.uk - which one have you got ??? Very confusing :/ |
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susan p
Gold Member
Scotland
915 Posts |
Posted - 12 Feb 2010 : 9:12:16 PM
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LOL Leigh Ann at the winnie the pooh bottle  xxx |
www.blackislearabians.com
The greatness of a nation and its moral progress can be judged by the ways its animals are treated
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Pashon2001
Platinum Member
3575 Posts |
Posted - 12 Feb 2010 : 9:17:45 PM
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| I'll look at the make of it tomorrow, I was VERY lucky, having been given it on a sort of permanent loan, it was a proper laboratory centrifuge, takes 16 x 30ml tubes. It would have cost a fortune in its day. They can be horrendously expensive, you need one that will take at least 10ml and preferably 20ml or larger tubes as its too fiddly otherwise. And a lot of re spinning to fit the whole collection in! I wish I knew how to 'make' boys or girls respectively. Also I think a lot of people would say the same when wanting that ONE special foal from a very special mare/stallion. |
www.jarvastud.com http://hocon.webs.com/ |
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Vicki M
Silver Member
England
294 Posts |
Posted - 12 Feb 2010 : 11:03:16 PM
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Fascinating subject.
I wish I had the time to get into all this. Just the experience would suit me.
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Vicki M
Al Karif Arabians |
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Topic |
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Starting young colts
